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KMID : 0648320090150030028
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Journal of The Korean Society of Hypertension
2009 Volume.15 No. 3 p.28 ~ p.35
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Salivary High Sensitive C-reactive Protein in Hypertension and Acute Coronary Syndrome
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Lee Sang-Rok
Son Hyun-Jin
Cho Yong-Gon
Cho Nam-Po
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Abstract
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Background: Human saliva contains a large number of proteins and peptides whose composition may be altered as a consequence of diseases, and so the analysis of saliva can provide some specific biomarkers. The purpose of this pilot study was to investigate the salivary proteomics and the level of highly sensitive C-reactive protein (hsCRP) in patients who suffer with hypertension (HTN) and acute coronary syndrome (ACS).
Methods: The study group was comprised of twenty four patients who received diagnostic coronary angiography. Those patients with conditions such as old age (> 60-year) or diabetes mellitus were excluded to avoid the secondary effects on saliva. First, a subgroup analysis was done according to the existence of HTN: group I (12 patients with HTN, 56.2±6.9 years, 6 male) and group II (12 patients without HTN, 52.8±4.7 years, 9 male). A second subgroup analysis was done according to the existence of ACS: group III (14 patients who had ACS, 54.1±5.9 years, 12 male) and group IV (10 patients who had stable angina, 54.9±6.5 years, 3 male). We compared the plasma and salivary hsCRP levels according to the subgroup analysis and we compared the salivary proteomics in the first subgroup (Ed note: first two subgroups? The last part of this sentence seems incomplete. Check it.)).
Results: The baseline clinical and cardiovascular risk factors and the laboratory findings were not significantly different between the first two subgroups. The baseline clinical and ardiovascular risk factors were not significantly different between the second two subgroups, except for gender (Group III: Group IV = 12 (85.7%): 3 (30%), p=0.01). The laboratory findings were not significantly different between the second two subgroups, except for the salivary hsCRP level (Group III: Group IV = 7.1±6.8 ng/mL: 2.5±1.7 ng/mL, p=0.028) and the serum neutrophil count (Group III: Group IV = 4525.6±337.7/μL: 3614.5±221.0 /μL, p=0.042). Salivary proteomic analysis that was done by performing two-dimensional polyacrylamide gel electrophoresis (2-DE) and mass spectrometry showed 9 different spots between the first two subgroups (three spots of kallikrein-1, two spots of pro2044 and two spots of albumin, poly-Ig receptor and haptoglobin 2). (ED note: the number of spots doesn’t add up.)
Conclusion: The salivary hsCRP level was increased in the patients who had ACS. Different patterns of proteomics were observed in the patients with HTN. Further investigations are needed to clarify the clinical possibility of observing these molecules on the salivary 2-DE of patients with HTN.
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KEYWORD
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Saliva, Hypertension, Proteomics, Coronary
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